Biosynthesis of β-tyrosine

Abstract

1. 1. An enzyme: tyrosine α,β-amino mutase was isolated from cells of Bacillus brevis Vm4. 2. 2. Tyrosine α,β-amino mutase forms β-tyrosine from L-α-tyrosine. 3. 3. The optimal conditions for the reaction are at pH 8.5 and 37°C. 4. 4. ATP is the only cofactor required for the enzyme activity. 5. 5. Tyrosine α,β-amino mutase is inhibited by compounds possessing α-keto groups and by carbonyl group binding agents. 6. 6. The enzyme was purified 125-fold by Sephadex G-200 fractionation and subsequent electrofocusing. 7. 7. The enzyme has a molecular weight of 75000 and an isoelectric point of 4.6.