Abstract
Cell-free extracts from Saccharomyces cerevisiae catalyzed the incorporation of glucosyl residues from UDP-[U- 14C]glucose into β-1, 3-glucans which contained a significant proportion of β-1, 6-glycosidic linkages. When GDP-[U- 14C]-glucose was used as substrate only trace amounts of glucose were incorporated. Activity of β-glucan synthetase was distributed among membrane and cell wall fractions, specific activity being higher in this latter. β-Glucan synthesized by membrane and cell wall fractions contained 0.6% and 2.5% of β-1, 6-glycosidic linkages respectively. A marked decrease in the activity of β-glucan synthetase occurred as the cells aged. Significant activity of glycogen synthetase was detected only in cells which had reached the stationary phase of growth.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - General Subjects
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