Biosynthesis, Characterization, Antibacterial Activity and Anticancer Effect of Silver Nanoparticles Using Anethum Graveolens Leaf Extract

Abstract

Nanoparticles are used in various fields of science, especially medicine. Advent of nanotechnology has led to significant development in disease diagnosis, treatment and drug delivery. Silver nanoparticles (Ag-NPs) play an important role in medical application, which makes them a viable alternative to common antibiotics. Amongst various methods, synthesis of Ag-NPs via green method has the advantage of being cost effective with no toxic agent. In this study, Spherical shape Ag-NPs with average size of 30 nm were synthetized using Anethum graveolens leaf extract as a green, cost-effective, non-toxic and environment-friendly source. Transmission electron microscopy (TEM), particle size analysis (PSA) and Fourier transform infrared (FT-IR) were performed to characterize synthesized Ag-NPs. The antibacterial activity of the synthetized Ag-NPs was evaluated against gram positive and negative bacterial pathogens. The minimum inhibitory concentration (MIC) at different concentrations of Ag-NPs were used to evaluate their antibacterial properties against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa pathogens. The results exhibited a desirable antibacterial property of Ag-NPs, suggested its usage as putative antibacterial agents. Moreover, the anticancer effect of green synthesized Ag-NPs was evaluated against MCF-7 lines and results showed that the cell viability is depended on the concentration of Ag-NPs. In short, this method provides a simple, cost effective and eco-friendly way to synthesis Ag-NPs which can be used as a suitable alternative to common antibiotics that use hazardous chemical agents, additionally, with anticancer effects against MCF-7 cells.