Biosynthesis, characterization, and determination of trace hydrogen peroxide of Organo-Cr(III) nanoparticles by Lysinibacillus sp. 4H

Abstract

The mechanism of microbial reduction of Cr(VI) has been widely reported; however, only a few studies have focused on Cr(VI) reduction products. In this study, a green synthetic pathway for the biosynthesis of Organo-Cr(III) nanoparticles using Lysinibacillus sp. 4H was investigated, and some properties of these nanoparticles were characterized, based on analysis using X-ray photoelectron spectroscopy, electron paramagnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy, among other techniques. The analyses revealed that the reduction product induced by Lysinibacillus sp. 4H may be amorphous Organo-Cr(III) nanoparticles with an irregular spherical structure (20–90 nm). Thermal characterization of the nanoparticles showed that they maintain a high residual mass (50.45%) at 700 °C, indicating high stability. In addition, the nanoparticles were capable of detecting trace amounts of hydrogen peroxide (H2O2), owing to their redox properties, such that the corresponding H2O2 concentrations could be accurately determined in a range of concentrations. This study provided novel insights and strategies regarding the use of nanoparticles to detect trace hydrogen peroxide concentrations in multiple fields.