Abstract
The present study highlights the biosynthesis of silver nanoparticles (AgNPs) using culture supernatant of Massilia sp. MAHUQ-52 as well as the antimicrobial application of synthesized AgNPs against multi-drug resistant pathogenic Klebsiella pneumoniae and Salmonella Enteritidis. Well-defined AgNPs formation occurred from the reaction mixture of cell-free supernatant and silver nitrate (AgNO3) solution within 48 h of incubation. UV-visible spectroscopy analysis showed a strong peak at 435 nm, which corresponds to the surface plasmon resonance of AgNPs. The synthesized AgNPs were characterized by FE-TEM, EDX, XRD, DLS and FT-IR. From FE-TEM analysis, it was found that most of the particles were spherical shape, and the size of synthesized nanoparticles (NPs) was 15–55 nm. EDX spectrum revealed a strong silver signal at 3 keV. XRD analysis determined the crystalline, pure, face-centered cubic AgNPs. FT-IR analysis identified various functional molecules that may be involved with the synthesis and stabilization of AgNPs. The antimicrobial activity of Massilia sp. MAHUQ-52 mediated synthesized AgNPs was determined using the disk diffusion method against K. pneumoniae and S. Enteritidis. Biosynthesized AgNPs showed strong antimicrobial activity against both K. pneumoniae and S. Enteritidis. The MICs of synthesized AgNPs against K. pneumoniae and S. Enteritidis were 12.5 and 25.0 μg/mL, respectively. The MBC of biosynthesized AgNPs against both pathogens was 50.0 μg/mL. From FE-SEM analysis, it was found that the AgNPs-treated cells showed morphological changes with irregular and damaged cell walls that culminated in cell death.
Highlights
Various metal nanoparticles (NPs) are synthesized due to their unique properties, such as electrical, optical, biological, catalytic, and magnetic characteristics and low-cytotoxicity and enhanced permeability [1,2,3]
Strain MAHUQ-52 was isolated from the rhizospheric soil of a banana plant and used for the biologically facile synthesis of AgNPs
The phylogenetic analysis based on the neighborjoining method revealed that strain MAHUQ-52 is grouped within the genus Massilia (Figure 1)
Summary
Various metal nanoparticles (NPs) are synthesized due to their unique properties, such as electrical, optical, biological, catalytic, and magnetic characteristics and low-cytotoxicity and enhanced permeability [1,2,3]. Among different metal NPs, silver nanoparticles (AgNPs) have drawn countless attention due to their various applications in different sectors as cancer therapeutics, biosensors, antibiotics, anti-inflammatory and drug delivery, etc. Many recent studies reported that biogenic AgNPs exhibited strong antibacterial activity against different pathogenic microorganisms [9,12,13]. Studies suggest that biosynthesized AgNPs have excellent anti-inflammatory and anti-cancer activities [5,6]. Fouda et al [7,8], biogenic AgNPs showed strong activity in degrading various toxic chemicals. Different physical and chemical methods have been applied for the synthesis of metal NPs. globally, it is realized that biological synthesis methods are safe, facile, and non-toxic compared to the expensive, toxic and dangerous chemical and physical processes [7,16]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
