Abstract
The aim of this research work was to create compelling strategy for biosurfactant generation by waste potato peels and pulp utilizing mutated B.subtilis DDU20161. Major parameters like creation of medium, fomentation, and pH were kept up in the investigation. The ideal biosurfactant yield by acetone extraction technique was 253.79 mg L-1 with biomass of 3.56 gL-1 at 40 h. The produced biosurfactant was characterised by studying surface tension, emulsification index, TLC, CMC and FTIR. The highest emulsification index was 75.05% in benzene, Critical micelle concentration(CMC) was found to be 0.028μM, and FT-IR study revealed presence of peptide bond at 3188cm-1 (=N-H). The results of stability study indicated that the produced biosurfactant is stable at 300-1000C without any significant change in surface tension properties of biosurfactant. The shaped biosurfactant was found to be stable in alkaline pH. The present work was focussed on developing a production methods utilizing acetone precipitation system.
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