Biosynthesis and Characterization of Silver Nanoparticles Using Barleria cuspidata Leaves Extract and Mechanism of Antimicrobial Action of Selected Human Pathogens

Abstract

Introduction: Nanoscience and nanotechnology are concerned in the invention and appliance of nanoparticles that can be used in diverse field. Silver nanoparticles (AgNPs), among other nanoparticles, have received considerable thought because of their inimitable properties.
 Objective: The intention of the current study was to quickly synthesize silver nanoparticles (AgNPs) using Barleria cuspidata leaf extract. The nanoparticles obtained have been characterized with a variety of characterization methods resembling UV-Visible Spectrum Analysis, Fourier Transform (FT) Infrared Spectrometry, Scanning Electron Microscopy, X-Ray Diffraction and tested against selected human pathogens.
 Results: Spectral studies confirmed the UV-Visible absorption spectrum of the Ag-NPs at a wavelength of 470 nm and a good crystalline nature with a face-centered cubic crystal structure using XRD spectrum. Surface topography and the presence of Silver in the prepared sample have been confirmed from SEM and EDX measurements. Various functional groups present in the sample have been examined using FT-IR spectroscopic analysis. A homogeneous dispersion of oval form nanoparticles with a usual size of 10µm was confirmed by visualization using SEM EDX.
 In addition, the biological activities of Ag nanoparticles showed significant results against anti-bacterial (E.coli, Staphylocouus aureus), and antifungal activity (Aspergillus niger, Candida albicans).
 Conclusion: Barleria cuspidata being a crucial medicinal plant widely used in the treatment of diabetic agent, the present study shows that the plant can be used as finest resource for synthesis of AgNPs for further research in the medical field.