Abstract
The present study aimed to synthesis of SNPs (Silver Nanoparticles) or (Green-Silver) in aqueous medium using leaf extracts of Ocimum sanctum L. The synthesized silver nanoparticles (SNPs) were characterized by the different techniques such as UV-vis Spectrophotometer, XRD, FTIR and TEM. The synthesis of silver nanoparticles were confirmed due to the SPR optical absorption band peak at ~440 nm by UV-vis spectrophotometer, nearly 15-45 nm in diameter with spherical in shape by TEM, high crystalline with the Bragg peaks of (111), (200), (220) and (311) plane as the predominant orientation by XRD and FTIR confirmed the presence of methoxy and allyl groups in SNPs. The reduction of Ag metal ions was performed due to the presence of various types of anti-oxidants in the leaves of Ocimum sanctum L. and each having a unique structure and functions. The formations of silver nanoparticles through aqueous medium were fairly stable and which can be incubated up to 4 months in refrigerator. At last, the antimicrobial activities of AgNPs were evaluated using different bacteria strain. It was found that, this biosynthesizing of silver nanoparticles may inhibit the all types of bacterial pathogenic organisms competently. This recent research study opens an innovative design to progress our understanding of how SNPs behave can be optimized to improve human antimicrobial activities.
Highlights
In the last decade there had been tremendous developments in nanotechnology science, which resulted in the establishment of several new techniques for research
The silver nitrate solution (AgNO3) was mixed with leaf extracts of Ocimum sanctum showed change of brown colour from transparent solution which confirmed the synthesis of silver nanoparticles
The reaction mixture was determined by UVvis from 0 till 45 min and formed surface plasmon resonace of silver nanoparticles (SNPs) peak centered near 440 nm (Figure 2) which indicated the reduction of AgNO3 into SNPs
Summary
In the last decade there had been tremendous developments in nanotechnology science, which resulted in the establishment of several new techniques for research. Nanotechnology has achieved the rate of combing all living organisms-plants, animal and microorganisms [1,2,3,4,5,6,7]. It includes multidisciplinary research interest like Biochemistry, Material Science, Polymer Science and etc. This has made nanotechnology indispensible in such diverse field as agriculture, industry, medicine, forestry and environmental management [8,9,10,11,12]. The active constituent of leave of the Ocimum sanctum L. is Eugenol (l-hydroxy-2-methoxy-4allylbenzene) which is mainly responsible for the therapeutic potentials of cardiovascular system, urinary system, reproductive system, immune system, gastric system, blood biochemistry, central nervous system and significance in various ailments in modern medicine [19,20,21]
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