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Abstract
The biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumulation ranging from 49.7 to 68.9% on cell dry weight (CDW) basis were observed when fatty acids ranging from octanoic acid (C8∶0) to oleic acid (C18∶1) were used as sole carbon and energy source. Molecular weight of the polymer was found to be ranging from 55.7 to 77.7 kDa. Depending on the type of fatty acid used, the 1H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability, having a thermal degradation temperature (T d) of 264.6 to 318.8 (±0.2) oC, melting temperature (T m) of 43. (±0.2) oC, glass transition temperature (T g) of −1.0 (±0.2) oC and apparent melting enthalpy of fusion (ΔH f) of 100.9 (±0.1) J g−1.
Highlights
The current increase in the utilization of polyhydroxyalkanoates (PHAs) in various industrial and biomedical applications is due to their biodegradability, compatibility, resorbability and piezoelectricity [1]
When fatty acid is fed to these bacteria, it passes through betaoxidation biosynthetic pathway to produce the PHA intermediates thereby losing two (2) carbon atoms per each cycle [15,16]
The biochemical analyses indicated a probability of 99.5% for the isolate to be identified as Pseudomonas putida
Summary
The current increase in the utilization of polyhydroxyalkanoates (PHAs) in various industrial and biomedical applications is due to their biodegradability, compatibility, resorbability and piezoelectricity [1]. Their diverse chemical properties have made them a subject of many research interests. The increasing demand of highly functionalized PHA for specialty applications, warrant the bio-prospecting of bacterial species capable of accumulating these biodegradable polymers. Several bacterial species known to accumulate PHA have been isolated from different ecological niches [3,4,5,6,7,8]. When fatty acid is fed to these bacteria, it passes through betaoxidation biosynthetic pathway to produce the PHA intermediates thereby losing two (2) carbon atoms per each cycle [15,16]
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