Biosynthesis and characterization of Eupatorium adenophorum and chitosan mediated Copper oxide nanoparticles and their antibacterial activity

Abstract

The biosynthesized metal oxide nanoparticles are of particular interest due to their wide applications in diverse fields. In the present study, copper oxide nanoparticles were successfully synthesized using chitosan and aqueous leaf extract of Eupatorium adenophorum. The green synthesized chitosan-plant extract mediated copper oxide (CPC) nanoparticles were characterized by Ultraviolet–visible (UV–Vis), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), Energy Dispersive X-ray Analysis (EDAX), High Resolution Transmission Electron Microscopy (HRTEM) and Selected Area Electron Diffraction (SAED). The FESEM and HR-TEM shows the synthesized nanoparticles structures as Nano flakes and Nano rods with size ranging between 30 nm–80 nm. EDAX shows the purity of the elemental constitutions. Antibacterial efficacy for the synthesized nanoparticles was tested against two-gram positive bacteria viz. Enterococcus faecalis and Staphylococcus aureus and two gram negative bacteria viz. Escherichia coli and Salmonella typhi The maximum zone of inhibition for E. faecalis, S. aureus, E. coli, S. typhi were 18 mm, 12 mm, 11 mm and 12 mm respectively. The synergic effects between chitosan and plant extract in determining the shape and size of the nanoparticles are well established.