Abstract

Bacterial nanocellulose (BNC) and polyhydroxyalkanoate (PHA) biopolymers were extracted using Bacillus velezensis BV-HSTU-FPP and Bacillus subtilis BS-HSTU-FPP strains which were isolated from fermented coconut water. Subsequently, bacterial nanocellulose and polyhydroxyalkanoate were used to synthesize biopolymer films along with CMC, gelatin and tween-80. 16S-rRNA sequencing was used to identify bacterial strains and structural properties of biopolymer films were determined using SEM, XRD, FTIR and TGA techniques. Functional and barrier properties of the films were determined using solubility, swelling degree, WVP, WCA, transparency and mechanical strength. XRD analysis showed that BNC (77.25 %) and PHA (68.93 %) films had higher crystalline properties than biopolymer-free film (57.91 %). Biopolymer films containing BNC showed high peak absorption at the O-H group, whereas PHA film was at -COO group as compared to biopolymer-free film. Additionally, films containing BNC and PHA increased thickness, WCA, tensile strength and elongation while decreased solubility, swelling degree and WVP than those of biopolymer free film. SEM analysis revealed biopolymer aggregation in both BNC and PHA films.Thermal stability and transparency of the biopolymer and non-biopolymer films were also comparable. The findings indicated that the BNC and PHA biopolymer-based films could have improved film properties that would be used to formulate sustainable packaging films.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.