Biosynthesis and Chaerchterization of Gold Nanoparticals by Using Local Serratia spp. Isolate

Abstract

Biological sources of bacteria ,fungi, and plants are playing a major role in the reduction of metallic nanoparticles such as gold, as it attributed as an eco-friendly and contributed in application in nanotechnology. This study include the biological synthesis of gold nanoparticles using the culture supernatant of local Serratia spp. isolate. Gold(III) chloride trihydrate ( HAuCl2) in concentration1× 10-³ M added to supernatant separately. Their respective supernatants were examined for ability to produce gold nanoparticles, The events happend was in a dark place at 37Cº. After 24 h, it was observed that the color of the solutions turned from pale yellow to dark purple. The gold nanoparticles were characterized by: UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) to ensure presence of different functional groups, respectively ,X-Ray Diffraction Analysis (XRD), finaly,scanning electron microscopy (FE-SEM) to determined AuNPs size and shape. Results: The gold nanoparticles were approximatly uniform in size 57.17 nm , triangle in shape and FTIR spectra revealed the presence of various functional groups in the gold nanoparticles which were also present in the bacterial extract. Conclusion: The current approach suggests that rapid synthesis of nanoparticles would be feasible in developing a biological process for mass scale production of gold nanoparticles.