Abstract
Biosynthesis for the preparation of antimicrobial silver nanoparticles (Ag NPs) is a green method without the use of cytotoxic reducing and surfactant agents. Herein, shape-controlled and well-dispersed Ag NPs were biosynthesized using yeast extract as reducing and capping agents. The synthesized Ag NPs exhibited a uniform spherical shape and fine size, with an average size of 13.8 nm. The biomolecules of reductive amino acids, alpha-linolenic acid, and carbohydrates in yeast extract have a significant role in the formation of Ag NPs, which was proved by the Fourier transform infrared spectroscopy analysis. In addition, amino acids on the surface of Ag NPs carry net negative charges which maximize the electrostatic repulsion interactions in alkaline solution, providing favorable stability for more than a year without precipitation. The Ag NPs in combination treatment with ampicillin reversed the resistance in ampicillin-resistant E. coli cells. These monodispersed Ag NPs could be a promising alternative for the disinfection of multidrug-resistant bacterial strains, and they showed negligible cytotoxicity and good biocompatibility toward Cos-7 cells.
Highlights
Drug-resistant infections are a major cause of death and have resulted in a serious risk to public health
The morphology and size of the Ag Silver nanoparticles (NPs) were further characterized by high-resolution Transmission electron microscopy (TEM) (HRTEM)
In order to investigate if the Ag NPs really affects the antibiotic-resistant bacterial cells, we evaluated the antibacterial activity of Ag NPs against ampicillin-resistant E. coli by colony-forming unit assay
Summary
Drug-resistant infections are a major cause of death and have resulted in a serious risk to public health. Increasing resistance to antimicrobial drugs is emerging as an urgent problem in medicine [1]. A number of strains of Staphylococcus aureus are resistant to methicillin and are the major cause of acquired infections in hospitals. Other antibiotic-resistant bacteria include penicillin-resistant Neisseria gonorrhoeae and multidrug-resistant Escherichia coli (E. coli) [2, 3]. The major mechanisms of resistance are increased efflux and the reduced absorption of antibiotics [4]. Another mechanism of drug resistance is the expression of enzymes that modify the molecular structure of antibiotics [5]. Much effort has been focused on developing
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