Biostructural chemistry of magnesium. Regulation of mithramycin-DNA interactions by Mg 2+ coordination

Abstract

Divalent magnesium promotes the selective binding of mithramycin to a cognate DNA sequence (5′-XXGCXX-3′) by a coupled mechanism requiring local conformational changes in the polynucleotide backbone and a structural rearrangement of the mithramycin dimer. Circular dichroism, 31P-NMR, and analysis of the topological change of supercoiled plasmid DNA by agarose gel electrophoresis support these conclusions and offer insight on the regulatory role of Mg 2+. Molecular modeling suggests that sequence selectivity arises from preferential coordination of Mg 2+ to d(GpC) domains in the minor groove of Z-type DNA. We suggest a specific structural role for the essential divalent magnesium ion, and propose a revision of the binding mechanism previously devised for the mithramycin/DNA complex. This revised structural model is entirely consistent with NMR results reported for the mithramycin and related chromomycin complexes, but previously interpreted in terms of an A-type configuration.