Biostatistical Relationship Between Apelin Expression and Radiosensitization in Nasopharyngeal Carcinoma Cells Based on Flow Cytometry and Cell Imaging

Abstract

Objective: To explore the biostatistical relationship between Apelin expression and radiosensitization in nasopharyngeal carcinoma (NPC) cells. Methods: The main operation of the experiment on NPC cells 6-10B and HNE2 include: colony formation, flow cytometry for radiotherapy, detection of cell proliferation according to the results of radiotherapy, protein immunoblotting for cells, detection of the effect of Apelin on the expression of hormones in cells by Western Blot method, immunofluorescence assay, electrophoresis, transmembrane closure, incubation of anti-cancer cells. The effects of Apelin on NPC cells were obtained by biostatistical analysis. Cells were scanned by a scanning electron microscope, the anisotropic analysis algorithm was used to analyze and compare the results of cell radiotherapy. Results: Apelin-13 inhibits the proliferation of NPC cells 6-10B and HNE2. Moreover, Apelin-13 can induce the overexpression of Cyclin D1 in NPC cells and promote the proliferation of NPC cells and inhibition of Caspase 3 expression and apoptosis of NPC cells. Apelin also has a significant effect on radiosensitization of NPC cells. Conclusion: Apelin controls the expression of Cyclin D1 and Casepase 3 cytokines. It can increase the apoptotic degree of NPC cells, inhibit the proliferation of NPC cells, and improve the radiosensitivity of NPC cells.