Bio-stabilization of toxic weeds (Xanthium strumarium and Lantana camara) implementing mono- and polyculture of Eisenia fetida and Eudrilus eugeniae.

Abstract

The present study investigates the synergistic impact of earthworms (Eisenia fetida and Eudrilus eugeniae) and microbes during vermicomposting of invasive weed phytomass (Xanthium strumarium and Lantana camara). This study aims introducing an onsite solution for weed control while producing valuable organic manure. Vermitransformation and detailed characterization of mono- (VC1, VC2, VC4, VC5) and polyculture (VC3, VC6) of X. strumarium and L. camara has been reported for the first time employing E. fetida and E. eugeniae. The study achieved 45.16 ± 2.48-76.73 ± 1.37% vermiconvertion rate. The pH, conductivity, and concentration of heavy metals are effectively stabilized. Furthermore, it observed a significant reduction in total organic carbon (TOC) alongside the augmentation of nitrogen, phosphorus, potassium, calcium, and other trace elements (Zn, Ni, Fe). The ash content, humification index, and C/N ratio analysis established the maturity of the vermicompost. The macronutrient enhancement in the vermicompost samples was recorded 1.5- to 2.47-fold for total N, 1.19- to 1.48-fold in available P, 1.1- to 1.2-fold in total K, and 1.1- to 1.18-fold in total Ca. The germination index reveals a significant reduction in phytotoxicity, suggesting the production of mature and suitable vermicompost for agricultural use. Evaluating mono- and polyculture techniques, the research highlights the superiority of E. fetida over E. eugeniae. Further, the earthworm population and biomass have significantly increased by the end of 60-day experimental trial.