Abstract
Transmission Electron Microscopy (TEM) and Energy Dispersive X-Ray (EDAX) microanalysis on selected metal bearing biomass samples of three microbial strains, BP 7 26 Arthrobacter sp., ER 121 Alkaligenes eutrophus, and AS 302 Pseudomonas mendocina, were performed in order to obtain information concerning the localisation of the metals Palladium, Silver, Yttrium, and Nickel in the different parts of the cells. Significant differences have been observed concerning the localisation of each metal. The results obtained by TEM and EDAX microanalysis show that although the strains examined present different characteristics, the observed localisation of the biosorbed metals could be metal rather than strain dependent. This work, combined with systematic experimental work on the determination of biosorption isotherms and metal uptake capacities for each of the above strains, as well as overall kinetics of the process, is a first step in the attempt to determine the active binding sites of the biomass types examined and, consequently, to elucidate the underlying mechanism of the sorption of the above metals by the biomass.
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