Abstract
Biosorptive removal of Reactive Black B (RBB) from aqueous solution by metabolically inactive Trichoderma harzianum (T. harzianum) was examined in this study. The individual and interactive effects of pH (2.0–10.0), temperature (25–45 °C), initial dye concentration (100–300 mg/L) and adsorbent dosage (0.1–0.5 mg/L) on the biosorption of RBB were evaluated using central composite design (CCD), and the mathematical model describing the biosorption process was developed. Maximum removal percentage (97.42 ± 1.3%) of RBB was attained at pH 5.75, temperature 37.12 °C, initial dye concentration 210.92 mg/L and biosorbent dosage 0.28 g/L. Suitability of the isotherms were determined using Langmuir, Freundlich and Temkin in this study, among which Freundlich isotherm was found suitable to equilibrium data. Kinetic analysis revealed that the adsorption data correlated well with pseudo-second-order kinetic model. The obtained thermodynamic data revealed that the RBB adsorption onto T. harzianum was exothermic and spontaneous nature during the process.
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