Abstract
Batik and textile industry is one of the sources of liquid waste derived from the staining process. One of the color substances commonly used is remazol brilliant violet which is an azotype substance that is difficult to be degraded in the environment and is toxic pollutants. A method that can be used to decolorize of synthetic dye is biosorption. Trichoderma viride can be used as biosorbent, since it is resistant to toxic compound. The immobilization of T. viride is applied to increase their stability and to minimize cellular damage. Calcium alginate can be used for immobilization matrix because it is affordable, non-toxic and biocompatible. This study focused on determining the optimum conditions on biosorption process of remazol brilliant violet using immobilized T. viride. Remazol brilliant violet solution were absorbed at pH 4, 5, 6, 7, 8, and 9, contact time for 10, 20, 30, 50, 60, 70, and 90 min, and the amount of adsorbent used were varied at 0.3; 0.4; 0.6; 0.8; and 1 g. The optimum condition was obtained at 60 min biosorption time, at pH 4 with the amount of adsorbent was 1 g, with percent of adsorption was 8.76%. The adsorption capacity of remazol brilliant violet using T. viride immobilized with Ca-alginate was 0.089 mg/g. FTIR spectra results indicated functional groups that involved in immobilization of T. viride were O-H and C=O carboxylic, while in biosorption process violet were C-H sp3, C=O carboxylic, C=O amide and C=C benzene.
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