Abstract

Lead removal efficiency of the bacterial strain, Bacillus subtilis (MTCC 2423) was tested with 200, 400, 600, 800 and 1000 ppm of lead in minimal broth for a period of ten days. Samples were tested for the level of lead every two days in each concentration and maximum removal was observed after six days of treatment. With the increase in lead concentration, both biomass and lead removal efficiency showed an increase. When tested with immobilized, dead and live cells, maximum removal was observed for immobilized cells. Among the sugars tested, monosaccharide sugars enhanced the biomass of B. subtilis during lead treatment and the results are discussed.

Highlights

  • Heavy metals employed in industries are hazardous to human beings and other organisms

  • The bacterial strain, B. subtilis was tested for the metal tolerance with a wide range of lead concentrations (50, 100, 500, 1000, 2000, 3000 and 4000 ppm)

  • The results indicate that after 24 hrs of incubation, B. subtilis grew well upto 1000 ppm concentration of lead

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Summary

Introduction

Heavy metals employed in industries are hazardous to human beings and other organisms. A sudden boost in the industrial activities has contributed quantitatively as well as qualitatively to the alarming increase in the discharge of metal pollutants into environmental sink, especially the aqueous environment [1,2]. Heavy metals reaching aquatic systems undergo food chain concentration and cause disorders in higher trophic levels. This fact renders the removal of heavy metals from aqueous solutions indispensable [3]. Intake of lead through external sources create a variety of surplus effects like elevation of blood pressure, damage of kidney and brain, infertility in men, learning disability and behavioral distraction in children and others within human body [7,8,9]

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