Abstract
In this study filamentous fungal biomass-loaded TiO(2) nanoparticles were used for the biosorption of lead(II) ions by flow-injection system coupled to flame atomic absorption spectrometry. The effects of pH, sample volume, loading and elution flow rates, eluent type and volume on the recovery of lead were investigated. Lead ions were sorbed on a biosorbent minicolumn at pH 4.0 followed by an elution step using 288 microL of 1.0 mol/L hydrochloric acid solution. The limit of detection was 0.78 microg/L. The validation of the described procedure was performed by the analysis of certified reference material (NRC-CNRC NASS-5 seawater). Finally, the presented biosorption procedure was applied to the determination of lead in tap water and seawater samples.
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