Abstract

The characteristics of potentiometric biosensors based on pH-sensitive field-effect transistors and the effect of reversible cholinesterase inhibition for determination of concentrations of aflatoxin B1 (AFB1) in agricultural products are optimized. Biologically active membranes were formed by crosslinking enzymes with bovine serum albumin on the surface of the transducers in an atmosphere of saturated vapors of glutaraldehyde.The optimal operating parameters of biosensors based on acetylcholinesterase from electric eel (KF 3.1.1.7) were determined, bioselective membrane with 1% enzyme content was selected, the concentration of acetylcholine chloride as a substrate for further inhibitory analysis of AFV1, and verifiability were received. The influence of sample preparation on the work of the biosensor was also studied.Walnuts, peas, as well as peanuts, wheat, oats and corn, specially infected by the mold fungus Aspergillus flavus at the Zabolotny Institute of Microbiology and virology of NAS of Ukraine, were used as samples for testing the developed biosensor. As a control, extracts obtained by the same method from the same substrates but not infected with Aspergillus were used.Experiments were carried out to quantify the content of AFV1 in the samples and to determine their concentrations.

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