Abstract
Lipases are enzymes responsible for the conversion of triglycerides and other esterified substrates, they are involved in the basic metabolism of a wide number of organisms, from a simple microorganism and to mammals. They also have broad applicability in many fields from which industrial biotechnology, the production of cleaning agents, and pharmacy are the most important. The use of lipases in analytical chemistry where it can serve as a part of biosensors or bioassays is an application of growing interest and has become another important use. This review is focused on the description of lipases chemistry, their current applications and the methods for their assay measurement. Examples of bioassays and biosensors, including their physical and chemical principles, performance for specific substrates, and discussion of their relevance, are given in this work.
Highlights
While standard analytical methods and devices for various assays are common in laboratories and specialized workplaces, a single device for reliable handheld assays is not able to cover the whole spectrum of analytes that are measured for diagnostic, environmental, technological and other purposes
Lipase activity assay is performed for many purposes and it is quite standard in microbiology and biochemistry laboratories
Lipases are a group of enzymes with a huge relevance for bioassays such as biosensors development
Summary
While standard analytical methods and devices for various assays are common in laboratories and specialized workplaces, a single device for reliable handheld assays is not able to cover the whole spectrum of analytes that are measured for diagnostic, environmental, technological and other purposes. Biological origin is the second compartment of a biosensor and this part is necessary for providing specificity to an analyte. Lipases are frequently used in biosensors construction because of their wide substrate specificity and good commercial availability. Molecules 2019, 24, x FOR PEER REVIEW isolation of a lipase source cell lipase immobilization pure lipase esterified analyte biosensor sensor acid + alcohol. This review is focused on the survey of actual literature in the field of lipase biosensors, bioassays. Focused on the survey of actual literature the fieldin of and similar tools and Discussion of their applicability and in suitability thelipase currentbiosensors, analytical bioassays andincluded similar in tools methods. A search in a recentofpaper the issue with citations is current analytical praxis is included in this work.
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