Biosensor for glucose and fructose by forming immobilized whole cell enzyme membrane containing Zymomonas mobilis cells and bonding to surface of pH electrode : Korea Adv. Inst. Sci. Technol. KR-9107824; 2 October 1991

Abstract

The metabolic pathways of the ethanol producing bacterium Zymomonas mobilis are modelled, simulated and compared with measured data obtained by 13C NMR and FIA (flow injection analysis). The modelling and simulation of either wild type or genetically manipulated metabolic pathways in the anaerobic bacterium is achieved by numerical solution of a system of ordinary differential equations. These equations describe the biochemical reaction network controlled by enzyme kinetics. The dynamic behaviour caused by extracellular changes of the substrate level in the fermentation broth is simulated as well as steady state conditions. Statements and predictions about intracellular reactions on growth conditions and genetically engineered cultures become possible. The gained models are based on assumptions concerning enzyme kinetics and their parameters both of them mostly unknown or only determined in vitro yet. By 13C NMR spectroscopy, stationary fluxes and metabolite concentrations can be determined in vivo and without any use of kinetic principles. Quick dynamic changes triggered by the external glucose level are measured by flow injection analysis. Thus, validation of parameters combined with kinetics and simulated metabolite concentrations can be achieved.