Abstract
A simple and low-cost hydrogen peroxide (H2O2) biosensor has been developed using a Yucca filamentosa plant leaf membrane in conjunction with an oxygen (O2) sensor. The leaf membrane contains hydrogen peroxidase which can catalytically decompose H2O2 to produce O2. The response rate was faster for a Yucca membrane (t90 response time is ≈14 s) than a Yucca membrane with the O2-permeable membrane (t90 response time is ≈200 s) and the sensitivity was much improved. The biosensor exhibited an excellent linear calibration range from 85 μM to 3.75 mM H2O2 (correlation coefficient r = 0.9999) with a detection limit of 15.1 μM; repeatability (1.024%, n = 10). The effects of pH and temperature on the response of the H2O2 biosensor have been studied in detail. The working life-time of the biosensor was good as it retains 86.7% of its initial sensitivity at 25 °C even after 2350 determinations of H2O2 sample solutions. It has been successfully applied to the determination of H2O2 concentration in some commercial samples.
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