Abstract
In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 μM with sensitivity of 0.624 μA/μM and the limit of detection (LOD) of 0.016 μM (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable.
Highlights
Phenol and substituted phenols are toxic chemicals that are released into the environment from industrial wastewater such as textile, pesticides, mining, dyes, and petrochemical and pharmaceutical industries
The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable
Scheme 1 illustrates theand possible mechanism for the preparation of graphene composited with gold nanoparticles (AuNPs) fabrication of electrochemical biosensor based on
Summary
Phenol and substituted phenols are toxic chemicals that are released into the environment from industrial wastewater such as textile, pesticides, mining, dyes, and petrochemical and pharmaceutical industries. Graphene has ideal and unique properties such as excellent conductivity, large surface area, good chemical stability, mechanical strength and high charge transport mobility [11] It has attracted interest significantly in several technological application fields including nanoelectronics, nanocomposite, biosensing and bioelectronics [12]. The large surface area of graphene along with electronic properties and good mechanical strength make them a promising support material for metal nanoparticles These unique criteria decrease the agglomeration of AuNPs and enhance their stability into graphene nanosheet matrix, the potential application in biosensing has been explored. Chit., where the designed nanocomposite sensing material showed enhancement in analytical performances such as good selectivity, long-term stability and high sensitivity for phenolic compounds detection without the involvement of other electron mediators. The developed biosensor was successfully applied for phenol detection in real samples
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