Biosensing technologies for Mycobacterium tuberculosis detection: status and new developments.

Lixia Zhou,Dinggeng He,Xiaoxiao He,Kemin Wang,Dilan Qin

doi:10.1155/2011/193963

Lixia Zhou, Dinggeng He + Show 3 more

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https://doi.org/10.1155/2011/193963

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Journal: Clinical & developmental immunology	Publication Date: Jan 1, 2011
Citations: 102	License type: CC BY 3.0

Affiliation: Hunan University

Abstract

Biosensing technologies promise to improve Mycobacterium tuberculosis (M. tuberculosis) detection and management in clinical diagnosis, food analysis, bioprocess, and environmental monitoring. A variety of portable, rapid, and sensitive biosensors with immediate “on-the-spot” interpretation have been developed for M. tuberculosis detection based on different biological elements recognition systems and basic signal transducer principles. Here, we present a synopsis of current developments of biosensing technologies for M. tuberculosis detection, which are classified on the basis of basic signal transducer principles, including piezoelectric quartz crystal biosensors, electrochemical biosensors, and magnetoelastic biosensors. Special attention is paid to the methods for improving the framework and analytical parameters of the biosensors, including sensitivity and analysis time as well as automation of analysis procedures. Challenges and perspectives of biosensing technologies development for M. tuberculosis detection are also discussed in the final part of this paper.

Highlights

Mycobacterium tuberculosis (M. tuberculosis) is a much dangerous pathogenic bacterium that causes tuberculosis (TB)—one of the leading causes of death from infectious diseases [1]
Many methods and techniques have been developed for rapid detection of M. tuberculosis, such as polymerase chain reaction (PCR) [6,7,8,9], latex agglutination [10], enzyme-linked immunosorbent assay (ELISA) [11,12,13], radiometric detection [14], genprobe amplified M
The piezoelectric quartz crystal (PQC) sensor is one of the new bioelectrochemical devices used for direct detection of M. tuberculosis

Summary

Introduction

Mycobacterium tuberculosis (M. tuberculosis) is a much dangerous pathogenic bacterium that causes tuberculosis (TB)—one of the leading causes of death from infectious diseases [1]. The development of portable, real-time, sensitive, rapid, and accurate methods for M. tuberculosis detection is essential to effectively prevent TB infection [19, 20]. The piezoelectric quartz crystal (PQC) sensor is one of the new bioelectrochemical devices used for direct detection of M. tuberculosis.

Results

Conclusion