Abstract
A novel self-assembled glucose biosensor based on graphene oxide (GO) was constructed by using 1-pyrenebutyric acid–N-hydroxysuccinimide ester (PANHS) as linking molecular. The stepwise self-assembly process was performed for PANHS anchoring in N,N-dimethylformamide (DMF) solvent and the further glucose oxidase (GOD) binding in aqueous solution, respectively. The molecular interactions and the morphologic properties were characterized by Fourier transform infrared spectroscopy (FTIR), field emission scanning electronic microscopy (FESEM), and atomic force microscopy (AFM). In addition, the quantitative loadings of anchored PANHS and GOD were well elucidated by surface plasmon resonance (SPR) measurements. The obtained novel glucose sensor exhibited satisfactory analytical performance to glucose: wide linear range (4.0×10−6 to 4.4×10−3M), fast response (10s), high sensitivity (40.5±0.4mAM−1cm−2), and low detection limit (2μM, S/N=3). Furthermore, the biosensor exhibited excellent long-term stability and satisfactory reproducibility.
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