Abstract
Biosensing of red blood cell-derived extracellular vesicles with the advanced bright-field light optical polarization microscopy
Highlights
Red blood cell (RBC)-derived extracellular microvesicles (EVs) are recently recognized key regulators of cell-to-cell cooperation, blood cell function, coagulation, and probably inflammation, proliferation and tissue repair [1, 2]
On the actual step of optical detection of the RBC-extracellular vesicles (EVs) we identified their number and relative size distribution, the methods allowed determining the morphology as mean shape and ultrastructure and measure the concentration of using original soft called advanced highly dynamic resolution capture system(R)
Hs-OCM images supported by yellowgreen light allow visualizing cell-free EVs without possibilities for assay their structure and measurement of their number
Summary
Red blood cell (RBC)-derived extracellular microvesicles (EVs) are recently recognized key regulators of cell-to-cell cooperation, blood cell function, coagulation, and probably inflammation, proliferation and tissue repair [1, 2]. Berezin et al (2017), Biosensing of Red Blood Cell-derived Extracellular Vesicles with the advanced Bright-Field light Optical Polarization Microscopy. The aim of the study was to compare a capture ability of conventional hs-OCM and advanced bright-field light optical polarization microscopy in detection and measurement of the RBC-EVs. Methods: The study was retrospectively evolved 26 patients with established stable coronary artery disease who were examined between May 2015 and November 2016. Using advanced bright-field light optical polarization microscopy associated with original soft allows distinguishing low-contrasted objects in details when we used monochromatic light with λ=370+30 nm with further math modelling. Conclusion: the advanced bright-field light optical polarization microscopy allows detecting clinically relevant properties of EV in wide ranges and could be determined a new much promising technique, which allows assaying EV in low cost
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