Abstract
Ornamental pineapple is a hard plant with significant landscaping value. Typically, conventional propagation is performed by clump division with low yields, and may even spread diseases. Plant tissue culture is viable, yielding plants with a high phytosanitary and genetic quality over a short time period. This study aimed to verify the in vitro multiplication of ornamental pineapple plants (Ananas comosus var. bracteatus L.) in different micropropagation systems, in association with BAP concentrations. Plants with about 2 cm were used, transplanted to the different treatments: bioreactor, natural ventilation and conventional micropropagation, combined with 3 BAP concentrations (0, 1 and 2 mg L-1). The basic medium used consisted of MS salts. The highest number of shoots and in vitro culture growth were obtained with the use of bioreactor and culture medium containing 2 mg L-1 BAP. The temporary immersion bioreactor allows air renewal inside the bottles, leading to a better performance of in vitro cultivation of ornamental pineapple, when compared to conventional micropropagation.
Highlights
Ornamental pineapple (A. comusus var. bracteatus L.) belongs to the Bromeliaceae family, and it is the most economical and the third most commercialized species in the world, grown in Thailand, Costa Rica, Brazil, the Philippines, Indonesia and India (FAO, 2013).A. comosus var. bracteatus is a native species widely used in landscape compositions to delineate areas or beds (OLIVEIRA et al, 2010); it is perennial and cultivated, appreciated for the beauty of its leaves and inflorescence
The treatments consisted of micropropagation systems, combined with different concentrations of benzylaminopurine - BAP (0, 1 and 2 mg L-1)
It can be observed that, in the absence of BAP, the conventional micropropagation (CM) and the natural ventilation system (NVS) were superior (22.5%) in relation to shoot length (SL), when compared to the plants maintained in the temporary immersion bioreactor (TI) (Table 1)
Summary
Ornamental pineapple (A. comusus var. bracteatus L.) belongs to the Bromeliaceae family, and it is the most economical and the third most commercialized species in the world, grown in Thailand, Costa Rica, Brazil, the Philippines, Indonesia and India (FAO, 2013).A. comosus var. bracteatus is a native species widely used in landscape compositions to delineate areas or beds (OLIVEIRA et al, 2010); it is perennial and cultivated, appreciated for the beauty of its leaves and inflorescence. Bracteatus is a native species widely used in landscape compositions to delineate areas or beds (OLIVEIRA et al, 2010); it is perennial and cultivated, appreciated for the beauty of its leaves and inflorescence. This species is vegetative propagated by clump division, which can spread diseases such as fusariosis (SILVA et al, 2007). Plants coming from tissue culture techniques are still very expensive and difficult to acquire This can be basically attributed to the production costs in the laboratory, especially with the intense use of labor and losses during acclimatization (MOREIRA et al, 2013)
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