Bioresonance According to Paul Schmidt (BaPS) and its Beneficial Effects on the Integrity of the Intestinal Barrier in vitro

Abstract

The intestinal epithelium, which is only one cell layer thick, has two essential tasks. The first is to create a physical barrier between the contents of the intestinal lumen and the rest of our body. The second is to ensure an efficient absorption of essential nutrients from the gut lumen and to produce mucus, anti-microbial peptides and cytokines with both protective and immune-regulatory properties. Thus, a reduced barrier function may have consequences, not only for intestinal, but also for systemic health. In this study we investigated whether the use of a Mini-Rayonex device, which has been developed to emit a bioresonance frequency spectrum according to Paul Schmidt (BaPS), might be able to promote and maintain the integrity of the intestinal barrier. Intestinal epithelial cells (IPEC-J2) were cultivated on microporous transwell plates to build up an intestinal barrier after exposure to a Mini-Rayonex device with the dipole antenna system (= verum). Corresponding control cultures were treated in the same manner but were exposed to a non-working Mini-Rayonex device without the dipole antenna system (= placebo). The transepithelial electrical resistance was measured as an indicator of intestinal barrier integrity. Thereafter, the achieved intestinal barrier from both Mini-Rayonex devices was exposed to 500 and 1,000 µM H2 O2 for 30 hours and the transepithelial electrical resistance was measured again. Moreover, the effect of both Mini-Rayonex devices (verum and placebo) on the regeneration process of intestinal epithelial cells at conditions of oxidative stress was investigated via examination of the closure of a cell-free area. Exposure of the cells to the actively emitting Mini-Rayonex device strengthened the intestinal barrier more than 30 % in comparison to the non-emitting Mini-Rayonex device. Moreover, the strengthened intestinal barrier was significantly more resistant by 20 % towards 500 µM H2 O2 and about 30 % towards 1,000 µM H2 O2 after exposure to the actively emitting Mini-Rayonex device than the “normal” intestinal barrier after exposure to the non-emitting device. In accordance with this observation was the result that the regeneration process after traumatization or injury of intestinal epithelial cells was increased by the actively emitting Mini-Rayonex device by more than 12 % in direct comparison with the non-emitting device. We conclude that the use of the actively emitting Mini-Rayonex device in vivo might also result in an improved intestinal epithelial barrier integrity, function and regeneration, which might improve and maintain well-being and systemic health.