Abstract
Among all environmental contaminants, industrial dye is one of the major pollutants of soil, water, and air. There are different chemical, physical, and biological methods for removal of all types of synthetic media from the environment. One common method is to utilize microorganisms like yeast, fungi, or bacteria. In this study, we identified a soil-derived microorganism and evaluated its efficacy for removal of industrial dye from wastewater. First, a strain of fungus isolated from various samples of soil was defined via colonial identification and DNA sequencing. Second, the bioremoval activity of the defined fungus (Rhizopus oryzae) in treating the water-soluble monoazo dye, Reactive Red 198, in synthetic media was evaluated using high-performance liquid chromatography. The effects of varying factors in processing, including dye concentration, fungal volume, time, and pH were determined. Under optimal conditions, a dye removal rate of 96% was obtained.
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