Abstract
Abstract A comparative study of the accumulation of Reactive Red 11 (RR11) and Acid Green 1 (AG1) dyes using live yeast culture Pichia pastoris (P. pastoris) was investigated. The changes in inoculum volume, pH, initial dye concentration and incubation temperature were studied. The maximum percentage of color removal efficiency, dye uptake capacity and bioaccumulation concentrations were found at pH 2. The maximum dried biomass concentration was found at pH 3. When the dye concentration increased, the percentage of color removal, specific growth rate and dried biomass concentrations decreased. However, the dye uptake capacity increased with an increase in the initial dye concentration. At an ambient temperature of 30 °C, the uptake efficiency for both the dyes was optimal The yeast biomass was characterized using scanning electron microscopic (SEM) image and fourier transform infrared (FTIR) spectral analysis. In this study, P. pastoris accumulated both dyes and was found to be more suitable for AG1 dye than RR11 dye.
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