Bioremediation of sulfonamides by a microalgae-bacteria consortium – Analysis of pollutants removal efficiency, cellular composition, and bacterial community

Abstract

Antibiotics in wastewaters (e.g., sulfonamides (SAs)) are not effectively removed by the conventional bacterial processes. In this study, a microalgae (Scenedesmus obliquus)-based process was evaluated for the removal of SAs. The maximum removal efficiency of sulfadiazine (SDZ) and sulfamethoxazole (SMX) by the consortium was 5.85% and 40.84%, respectively. The lower SDZ biodegradation efficiency could be due to the difference in the lipophilic degree related to cell binding. The presence of SAs did not significantly inhibit the biomass production of the consortium (1311–1952 mg/L biomass) but led to a 36–51% decrease in total polysaccharide content and an increase in microalgae’s protein content, which caused granule formation. The presence of SMX and SDZ resulted in an increase in lipid peroxidation activity with a 6.2 and 23.5-fold increase in malondialdehyde content, respectively. Rhodobacter and Phreatobacter were abundant in the consortium with SAs’ presence, while alinarimonas, Catalinimonas and Cecembia were seen in their absence.