Abstract
When oil spill occurs, it poses serious toxic hazards to all forms of life. Mixed culture of <em>Burkholderia cepacia</em> and <em>Corynebacterium sp</em> isolated from refinery sludge using selective enrichment technique was used for bioremediation of refinery wastewater in a laboratoryscale bioreactor. Physicochemical parameters of both raw and treated water were as determined and compared with Federal Environ - mental Protection Agency (FEPA-limit, Abuja, Nigeria) to asses the efficiency of the bioremediation process. Each of the bacterium was screened for the presence of plasmid DNA and for the involvement or otherwise of plasmid in the bioremediation of wastewater. The immobilised cells showed percentage decrease in chemical oxygen demand (97%), biochemical oxygen demand (94%), phenol (98%), total petroleum hydrocarbon (79%), oil and grease (90%) of the refinery waste water after 20 days of treatment while their transconjugants showed the multiplicative effect by achieving the same percentage after 10 days of treatment. Therefore, the findings revealed that bioaugmentation of wastewater using transmissible catabolic plasmid will enhance efficiency of the bioremediation by spreading the plasmid among indigenous microbial community either through horizontal gene transfer or transformation.
Highlights
Collection of the wastewater SamplesThe wastewater samples from different concrete wastewater reservoirs in Kaduna Refinery & Petrochemical Company (KRPC; Kaduna, Nigeria) were collected into four litres plastic bottles and stored in an ice block cooler and transferred to the laboratory immediately for analysis.[18,19] All the collected samples were preserved in accordance with guidelines and International Standards
Acknowledgements: the authors thank the Board of Research and the Department of Microbiology, Ahmadu Bello University, Zaria for providing funds
The pH values varies between (8.0-6.55) while COD, phenol and oil and grease in raw waste water are 875 mg/L, 68.13 mg/L and 43.20 mg/L respectively while final level obtained in both immobilised cell treated wastewater and transconjugant mented with 1% crude oil
Summary
The wastewater samples from different concrete wastewater reservoirs in Kaduna Refinery & Petrochemical Company (KRPC; Kaduna, Nigeria) were collected into four litres plastic bottles and stored in an ice block cooler and transferred to the laboratory immediately for analysis.[18,19] All the collected samples were preserved in accordance with guidelines and International Standards. Wastewater released by petrochemical change.[17] The present work identified mixed Mixed culture of Burkholderia cepacia and industries are characterized by the presence culture of crude oil degrading Burkholderia Corynebacterium sp that showed degradative of large quantity of polycyclic and aromatic cepacia and Corynebacterium sp isolated from potential during mineralization of Bonny Light hydrocarbons, phenols, metal derivatives, sur- refinery oil sludge and their ability to transfer crude oil were immobilised on agar-agar as folface active substances, sulphides, naphethy - plasmid to the indigenous bacteria in the lows: agar-agar solution and inoculi for immolenic acids and other chemicals.[1,2] ecosystem for the preparation of bacterial bilisation were prepared separately as ; The process of refining crude oil consumes blend that could be used in bioremediation of Fifty mL of each of the inoculi was inoculated large amounts of water. Phosphate buffer was decanted and the cubes were washed with sterile distilled water 3 to 4 times before used.[24] pended with BH medium until OD600 was equivalent to 1.0
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