Abstract
The biodegradation of pymetrozine pesticide was investigated with Pleurotus eryngii white rot fungus (WRF). The biomass was characterized by Fourier-transform infrared spectroscopy (FTIR) and scanning electrode microscopy (SEM) to verify surface functionality and morphology. FTIR results revealed fungal biomass is rich with multiple functional groups while SEM analysis showed that the surface of fungal biomass is irregular, porous, rough and heterogeneous. Bioremediation of pymetrozine with Pleurotus eryngii was monitored using ultraviolet-visible spectroscopy. The Box Bhenken Design optimization showed that Pleurotus eryngii degrades 93.3% of 0.20 mM pymetrozine at pH 5. Gas chromatography–mass spectrometry (GC-MS) was carried out to identify major metabolites formed during biodegradation of pymetrozine. Based on these results, pymetrozine was transformed to nicotinaldehyde and 4-amino-6-methyl-4,5-dihydro-2H- [1,2,4] triazine-3-one (AMDT).
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