Abstract
Melanised chicken feather wastes are great resources of amino acids and oligopeptides which can be biodegradable by the bacterial keratinase enzyme. Biodegradation of melanised chicken feathers waste directed by P. woosongensis TKB2 cells was successfully carried out in 8 L EYELA-MBF-800ME laboratory scale fermentor. The fermentation processes were conducted at 30 °C, 700 rpm agitation speed and 0.7 vvm air flow rate in a basal medium (%, w/v, 0.05% K2HPO4; 0.025% MgSO4; 0.02% CaCO3 and 5% NaCl) containing 1.0 % (w/v) melanised feathers. Highest net levels of released feathers hydrolysis end products like soluble proteins of 4.15 mg/mL and an adequate level of free amino acids (isoleucine>serine>alanine>arginine>histidine>vali ne>phenylealanine>leucine>tyrosine), keratinolytic protease activity (71.68 UmL-1) and 98.8 % feather degradation capability in the fermentor were greatly comparable to those of shake flasks. The crude enzyme showed optimum activity against Anopheles stephensi larvae in native water at pH 8.0 and 50 °C (LD50-0.60 UmL-1 at 48 h). Therefore, the studied keratinase can be used as an effective mosquitocidal agent.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
