Bioremediation of Hydrocarbon Using Bacterial Lipase from Waste Biomass

Abstract

Hydrocarbons originated from automobile oils are significant polluting agents of the soil. Lipase-mediated catalytic degradation of hydrocarbons from this used automobile oil is attempted in the present approach. Lipase was produced from peanut oil industry waste biomass using thermophilic alkaline-tolerant Pseudomonas sp. S1 obtained from sediment of Taptapani hot spring, Odisha. The experiments were designed as per central composite design, which is response surface methodology, to study the effect of the parameters and their interaction on the activity. A quadratic regression was found to be best with the experimental data, R2 = 0.899741 and the maximum lipase production of 51.3 IU/g was predicted from peanut oil seed waste biomass at an optimum pH of 8.31, temperature of 50.58 °C, moisture content of 50.8%, and biosurfactant content of 1.051 mg. The significant interaction of pH and moisture content with biosurfactant during lipase production was observed through the contour plots and 3D surface responses. Pseudomonas sp. S1 could degrade 92.6% of the used automobile oil after incubation for 24 h under optimum pH of 8.31 and temperature of 50.58 °C. Degradation of petroleum hydrocarbons by this thermophilic isolate could be effective due to the ability of lipase to utilize the used automobile oils as sole source of carbon and energy.