Abstract
Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases.
Highlights
The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, confirming that cooking oil wastes can be bioremediated using orange lipases
It was possible seeing that the experiments using crude soybean oil and soybean oil waste showed different fatty acids ester (FAE) releases in the core and commercial lipase treatments. These results indicate that the lipolytic extracts were able to modify the soybean oil, both when it was raw and after it was subjected to prolonged heating
It is known that orange-derived lipases differ from other vegetable sources in weight and isoform variety, since the plant lipases in most studies weight 36–40 kDa up to 60 kDa [26, 28,29,30]
Summary
The aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. The aim of the current study was to analyze the effect of cooking oil waste bioremediation through hydrolysis and transesterification reactions catalyzed by lipases obtained from orange and commercial wastes
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
