Abstract
The clay with high oil content form soil lumps, which is hard for microbes to repair. In this paper, the bioaugmentation and biostimulation technology were applied to improve the bioremediation effect of the soil with high oil content, that modified by local cow dung and sandy soil, the ecological toxicity of the soil after restoration was further analyzed. After 53 days of bioremediation, the degradation efficiency with respect to the total petroleum hydrocarbons (TPH) content reached 76.9% ± 2.2%. The soil bacterial count of M5 group reached log10 CFU/g soil = 7.69 ± 0.03 and the results were better than other experimental groups. The relative abundances of petroleum-degrading bacteria added to M5 remained high (Achromobacter 9.44%, Pseudomonas 31.06%, and Acinetobacter 14.11%), and the proportions of some other indigenous bacteria (Alcanivorax and Paenibacillus) also increased. The toxicity of the bioremediated soil was reduced by seed germination and earthworm survival experiments.
Highlights
Natural attenuation (NA): 100 g distilled water + 385 g modified clay c (45 wt% clay with high oil content, 55 wt% sandy soil)
In view of the characteristics of oil-contaminated clay, this paper proposes a simple and effective bioaugmentation and biostimulation technology, which was applied to modified petroleum-contaminated clay to reduce oil content and viscosity, and repair the oil on the clay particles
The toxic effect on bacteria will increase if the content of petroleum in soil is higher than 6%15
Summary
Natural attenuation (NA): 100 g distilled water + 385 g modified clay c (45 wt% clay with high oil content, 55 wt% sandy soil). Biostimulation-1 (BS-1): 100 g nutrient solution + 385 g modified clay c (45 wt% clay with high oil content, 55 wt% sandy soil). Bioaugmentation (BA): 75 g distilled water 385 g modified clay d (45 wt% clay with high oil content, 45 wt% sandy soil, 10 wt% cow dung) + 25 g bacterial solution. Biostimulation (BS) + bioaugmentation (BA): 75 g nutrient solution + 385 g modified clay d (45 wt% clay with high oil content, 45 wt% sandy soil, 10 wt% cow dung) + 25 g bacterial solution. The ecological restoration ability of the modified soils was evaluated to ensure the vegetation cover in the later stage
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
