Bioremediation of chlorimuron-ethyl-contaminated soil by Hansschlegelia sp. strain CHL1 and the changes of indigenous microbial population and N-cycling function genes during the bioremediation process

Abstract

Long-term and excessive application of the herbicide chlorimuron-ethyl has led to soil degradation and crop rotation barriers. In the current study, we isolated bacterial strain Hansschlegelia sp. CHL1, which can utilize chlorimuron-ethyl as its sole carbon and energy source, and investigated its application in soil bioremediation. Indigenous microbial populations and N-cycling function in the soil were also investigated during the bioremediation process by monitoring the copy numbers of bacterial and fungal marker genes, as well as N-cycling functional genes (nifH, amoA, nirS, and nirK). Results showed that >95% of chlorimuron-ethyl could be degraded within 45 days in soils inoculated with CHL1. Inoculation at two time points resulted in a higher remediation efficiency and longer survival time than a single inoculation. At the end of the 60-day incubation, the copy numbers of most indicator genes were recovered to the level of the control, even in the single-inoculation soils. A double inoculation was necessary for recovery of nifH. However, the abundance of nirK and ammonia-oxidizing bacterial genes were significantly inhibited regardless of inoculum. The results suggested that CHL1 is effective for the remediation of chlorimuron-ethyl-contaminated soil, and could partially reduce the toxic effects of chlorimuron-ethyl on soil microorganisms.