Abstract
Bio-refining of bilberry pomace using innovative technologies such as microwave hydro diffusion and gravity extraction (MHG) and ultrasound assisted extraction (UAE) with different concentrations of ethanol/water as the solvent was established. Bead milling was also utilized in this sequential extraction scheme to remove the lipophilic fraction. Solubility index of target polyphenols was predicted using a computational tool (COSMO-RS) and compared to experimental results obtained by in vitro antioxidant activity assessments. MHG extracts obtained using microwave power of 2 W/g had the highest Folin-Ciocalteu reducing capacity (43.46 ± 0.48 mg GAE/g of extract), total flavonoid (4.17 ± 0.04 mg QE/g of extract), total monomeric anthocyanin content (12.19 ± 0.13 mg D3GE/g of extract) and radical scavenging capacity (22.64 ± 2.23 mg TE/g of extract). In UA ethanol/water extracts the highest flavonoid (10.41 ± 0.08 mg QE/g of extract) and anthocyanin content (12.19 ± 0.51 D3GE mg/g of extract) was present in e...
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