Abstract
Large volumes of treated process water are required for protein extraction. Evaporation of this water contributes greatly to the energy consumed in enriching protein products. Thin stillage remaining from ethanol production is available in large volumes and may be suitable for extracting protein rich materials. In this work protein was extracted from ground defatted oriental mustard (Brassica juncea (L.) Czern.) meal using thin stillage. Protein extraction efficiency was studied at pHs between 7.6 and 10.4 and salt concentrations between 3.4 × 10-2 and 1.2 M. The optimum extraction efficiency was pH 10.0 and 1.0 M NaCl. Napin and cruciferin were the most prevalent proteins in the isolate. The isolate exhibited high in vitro digestibility (74.9 ± 0.80%) and lysine content (5.2 ± 0.2 g/100 g of protein). No differences in the efficiency of extraction, SDS-PAGE profile, digestibility, lysine availability, or amino acid composition were observed between protein extracted with thin stillage and that extracted with NaCl solution. The use of thin stillage, in lieu of water, for protein extraction would decrease the energy requirements and waste disposal costs of the protein isolation and biofuel production processes.
Highlights
Brassica spp. oilseeds are grown throughout the world as sources of vegetable oil and protein-rich animal feed (Henriksen et al 2009,)
Maximum protein extraction efficiency was achieved at the highest pH and NaCl concentration employed (10.4 and 1.2 M, respectively) (Table 1)
Protein extraction using thin stillage and sodium chloride solution Efficiency of protein extraction The efficiency of protein extraction may be affected by the presence of compounds, such as divalent cations, which are found in industrial Thin stillage (TS) (Ratanapariyanuch et al 2011) but not in the NaCl solution
Summary
Brassica spp. oilseeds are grown throughout the world as sources of vegetable oil and protein-rich animal feed (Henriksen et al 2009,). TS is processed by drying than added to distillers dried grains (DDG) to produce DDG with solubles (DDGS). The latter is used in animal feeds. To overcome the losses in energy for this process several strategies have been proposed including feeding wet distiller’s grains with solubles. This has the advantage of decreasing the cost of drying but necessitates transporting water with the feed product to the animals. In addition the wet products may not be suited for storage
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