Biorefinery design with combined deacetylation and microwave pretreatment for enhanced production of polyhydroxyalkanoates and efficient carbon utilization from lignocellulose

Abstract

Lignocellulosic biomass has emerged as a sustainable and economically viable feedstock for polyhydroxyalkanoate (PHA) production. However, it has seldom been used as part of an integrated process for effective carbon utilization. An innovative combined deacetylation and microwave pretreatment strategy (DEA-MW) and a wild-type Cupriavidus necator strain with the potential to utilize a lignocellulose-based carbon source and PHA biosynthesis were selected to set up the module needed to enable a new sustainable platform for biomass pretreatment and waste carbon valorization. DEA-MW pretreatment was introduced to mitigate the concentration of inhibitory acetic acid and phenolic compounds in the biomass hydrolysate. This pretreatment enhanced deacetylation and delignification, resulting in 95.50 % deacetylation and 45.30 % delignification. When DEA-MW pretreated rice husk biomass was coupled with microbial fermentation, C. necator promoted the fermentability of the rice husk hydrolysate. With a 3 g/L of levulinic acid from hydrolysis, as the sole carbon source, we achieved a concentration of PHA 440 mg/L with a content of 65 %. We also successfully recovered 72.22 % of lignin using a combined DEA-MW pretreatment. A detailed structural analysis showed that β-O-4, β–β, and β-5 linkages had been preserved in the recovered lignin. This study has shown that DEA-MW pretreatment plays a role in promoting biomass conversion and enhancing PHA production and opens up new possibilities for efficient and cost-effective lignocellulose-based biorefinery processes.