Bioreducible cationic micelle/DNA multilayered films for localized gene delivery

Abstract

Summary Multilayered films composed of bioreducible cationic micelles, formed from amphiphilic disulfide-containing poly(amidoamine)s, and DNA were prepared via layer-by-layer assembly for localized gene delivery. The thickness of cationic micelles/DNA films with 10 bilayers increased with increasing the grafting degree of alkyl groups in poly(amidoamine)s. Meanwhile, films with 10 bilayers prepared from bioreducible polycations with 28% of alkyl side chains, showed the fastest release of DNA in the presence of 2.5 mM glutathione and highest transfection efficiency when 293T cells were cultured on the surface of films.