Abstract
Kinetic modeling of biomass and lactic acid production by Enterococcus faecalis SLT13 have been developed during batch culture in M17 and Hydrolyzed Cheese Whey (HCW) in 2 L and 20 L bioreactors. The specific growth rate μmax was higher in 20 L bioreactor (1.09 h−1); however, the maximum specific lactic acid production rate qpmax and maximum specific sugar utilization rate qsmax were higher in 2 L bioreactor. Biomass and sugar utilization were affected by lactic acid inhibition in HCW. No effects of substrate inhibition have been observed. Substrate limitation of biomass has been observed on HCW in 20 L bioreactor; the substrate limitation constant for biomass Ksx was 4.229 g/L. Substrate limitation of sugar consumption has been observed on M17 in 2 L bioreactor; the substrate limitation constant for sugar consumption Kss was 2.73 g/L. Compared to experimental data, the model provided good predictions for biomass, sugar consumption, and lactic acid production.
Highlights
The lactic acid and its derivative polylactic acid (PLA) market appears among the most important markets of the chemical industry. is market is in clear progress and it is predictable to attain USD 3.82 billion and 5.16 billion on 2020 for the lactic acid and the PLA, respectively. is growth is due to several reasons: the increasing request for the eco-friendly products, the increasing prices of products derived from the petrochemistry, and the limited reserves of fossil energy [1]
Effect of Culture Media and Scale-Up on Lactic Acid and Biomass Productivity. is study is conducted to evaluate the effect of scale-up on growth and lactic acid production by Enterococcus faecalis SLT13. e fermentation was first carried out at laboratory scale, in 2 L bioreactor, than at semipilot scale, in 20 L bioreactor
2 L bioreactor cultures were conducted in M17 and Hydrolyzed Cheese Whey (HCW) under controlled conditions, while only HCW was used for 20 L bioreactor; the same parameters were maintained
Summary
The lactic acid and its derivative polylactic acid (PLA) market appears among the most important markets of the chemical industry. is market is in clear progress and it is predictable to attain USD 3.82 billion and 5.16 billion on 2020 for the lactic acid and the PLA, respectively. is growth is due to several reasons: the increasing request for the eco-friendly products, the increasing prices of products derived from the petrochemistry, and the limited reserves of fossil energy [1]. Two processes are used to produce industrially lactic acid: chemical synthesis and microbial fermentation. With increasing interest in the industrial application of lactic acid and PLA, different kinetic models have been developed in order to study the growth and lactic acid production by different species of LAB. Is kind of model relates cell growth and lactic acid production by including two coefficients: growth-associated coefficient (α) and a non-growth-associated coefficient (β). Unstructured kinetic models such as the Gompertz equation have been used [20]. Is study is conducted to find the most suitable model that describes biomass and lactic acid production by Enterococcus faecalis SLT13 in batch cultures in a medium prepared with hydrolyzed cheese whey and in a synthetic medium (M17). Is study is conducted to find the most suitable model that describes biomass and lactic acid production by Enterococcus faecalis SLT13 in batch cultures in a medium prepared with hydrolyzed cheese whey and in a synthetic medium (M17). e effects of culture media and scale-up from 2 to 20 L bioreactors on the kinetic parameters were studied
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