Abstract
BackgroundSodium butyrate (NaBu) is not only known to inhibitproliferation but also to increase the specific productiv-ity in cultivation of Chinese hamster ovary (CHO) cells[1] – the most commonly used mammalian cell line forpharmaceutical protein production [2]. So far, little isknown about the underlying mechanisms and genes thatare affected by butyrate treatment. Besides the proteo-mic approach to unravel proteins involved in the pro-cesses, the analysis of transcriptomes presents anotherpromising method. Here we show an application of ourCHO cDNA microarray to identify genes associatedwith increased productivity during cultivation of CHOcells under sodium butyrate treatment.Materials and methodsFour batch cultivations of CHO DP-12 cells (clone #1934, ATCC CRL-12445) were performed in 2 L bior-eactor systems under pO
Highlights
Sodium butyrate (NaBu) is known to inhibit proliferation and to increase the specific productivity in cultivation of Chinese hamster ovary (CHO) cells [1] – the most commonly used mammalian cell line for pharmaceutical protein production [2]
The maximum antibody concentration of the control culture was 110 mg/L whereas cells treated with NaBu reached a maximum of 175 mg/L antibody. 72 hours after addition of NaBu the specific antibody production rate was increased by a factor of 3.6 (NaBu culture: 4.5 pg/(cell·d)) compared to control culture (1.2 pg/(cell·d))
From a total of 1461 genes found to be differentially expressed under NaBu treatment, 771 genes were upregulated and 690 genes were downregulated
Summary
Sodium butyrate (NaBu) is known to inhibit proliferation and to increase the specific productivity in cultivation of Chinese hamster ovary (CHO) cells [1] – the most commonly used mammalian cell line for pharmaceutical protein production [2]. Little is known about the underlying mechanisms and genes that are affected by butyrate treatment. Besides the proteomic approach to unravel proteins involved in the processes, the analysis of transcriptomes presents another promising method. We show an application of our CHO cDNA microarray to identify genes associated with increased productivity during cultivation of CHO cells under sodium butyrate treatment
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