Abstract
The polymerase chain reaction (PCR) technique has been adopted to identify the sex of preimplantation Indian Zebu and crossbred cattle embryos rapidly. PCR was used to amplify Y chromosome specific repeat sequences from bisected embryos. In the present study, four different primer pairs were utilized for the sexing of each embryo. The first oligonucleotide primer pair, the bovine satellite specific primer, recognizes sequences that are amplified both in males and females, while the other three bovine Y chromosome specific primer pairs amplified the Y chromosome specific sequence in males only. The sex of each embryo was confirmed by using different Y chromosome specific primers. Sex determination by PCR was found to be accurate and rapid enough to allow transfer of embryos of known sex without the need for cryopreservation.
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