Bioprosthetic mesh of bacterial cellulose for treatment of abdominal muscle aponeurotic defect in rat model.

Abstract

The use of meshes for treatment of hernias continues to draw attention of surgeons and the industry in the search of an ideal prosthesis. The purpose of this work is to use meshes manufactured from bacterial cellulose, evaluate their organic tissue interaction and compare with an expanded polytetrafluorethylene (ePTFE's) prosthesis used to repair acute defect of muscle aponeurotic induced in rats. Forty-five male Wistar rats were classified using the following criteria: (1) surgical repair of acute muscle aponeurotic defect with perforated bacterial cellulose film (PBC; n=18); (2) compact bacterial cellulose film (CBC; n=12) and (3) ePTFE; (n=15). After postoperative period, rectangles (2×3cm) including prosthesis, muscles and peritoneum were collected for biomechanical, histological and stereological analysis. In all cases, the maximum acceptable error probability for rejecting the null hypothesis was 5%. Between PBC and CBC samples, the variables of strain (P=0.011) and elasticity (P=0.035) were statistically different. The same was found between CBC and ePTFE (elasticity, P=0.000; strain, P=0.009). PBC differed from CBC for giant cells (P=0.001) and new blood vessels (P=0.000). In conclusion, there was biological integration and biomechanical elasticity of PBC; therefore, we think this option should be considered as a new alternative biomaterial for use as a bio prosthesis.