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Abstract
BackgroundWild-type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has to be determined. In this study, wild-type ethanologenic yeasts isolated from a secondary effluent were assessed for their capability to ferment saccharified microalgae sugars.ResultsYeast species in wastewater were identified sequencing the Internal Transcribed Spacers 1 and 2 regions of the ribosomal cluster. Concurrently, microalgae biomass sugars were saccharified via acid hydrolysis, producing 5.0 ± 0.3 g L−1 of fermentable sugars. Glucose consumption and ethanol production of yeasts in hydrolyzed-microalgae liquor were tested at different initial sugar concentrations and fermentation time. The predominant ethanologenic yeast species was identified as Candida sp., and glucose consumption for this strain and S. cerevisiae achieved 75% and 87% of the initial concentration at optimal conditions, respectively. Relatively similar ethanol yields were determined for both species, achieving 0.45 ± 0.05 (S. cerevisiae) and 0.46 ± 0.05 g ethanol per g glucose (Candida sp.).ConclusionOverall, the results provide a first insight of the fermentation capacities of specific wild-type Candida species, and their potential role in ethanol industries seeking to improve their cost-efficiency.
Highlights
Wild-type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has to be determined
Analyses in this study revealed that ethanologenic wild-type yeast thrive in municipal wastewater and certain species were able to effectively produce ethanol using hydrolyzed-microalgae liquor
Among the different non-Saccharomyces yeast species isolated from a secondary wastewater effluent, only Candida sp. yeast species exhibited similar ethanologenic behavior to S. cerevisiae S288C
Summary
Wild-type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has to be determined. Ethanol is considered a cleaner alternative due to its high biodegradability, low greenhouse gas emissions, up to 96% less than fossil fuels, and null toxicity [8,9,10,11]. It is miscible with gasoline and can be used as an oxygenated portion in spark-ignition engines to reduce CO2 emissions [12, 13]. Ethanol fuel is mostly produced from either edible-crops, called first-generation ethanol, or agricultural residues, known as second-generation ethanol [11, 14, 15]
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